Curated OER
Determining Cutting Site Locations
Students explore DNA restriction mapping. They cut pieces of paper into lengths representing those produced when specific enzymes are used to cut a strand of DNA.
Teach Engineering
DNA Forensics and Color Pigments
Use food coloring in electrophoresis. The last segment in a four-part series mimics DNA fingerprinting by using chromatography. Teams conduct chromatography on food coloring to find colors that use similar pigmentation in their makeup.
Teach Engineering
Restriction Enzymes and DNA Fingerprinting
Show your class why restrictions aren't always a bad thing. In the third segment of a four-part series, the instructor develops the idea of restriction enzymes. Pupils learn how scientists use restriction enzymes in DNA analysis and DNA...
Curated OER
Using a Controlled Experiment to Identify Two Unknown Plasmids
Student demonstrate knowledge of recombinant DNA techniques (restriction enzyme digest, gel electrophoresis, and staining gel). They demonstrate skills needed to complete a gel electrophoresis and interpret a stained gel. Pupils solve...
Curated OER
Biotechnology
The concepts here cover the gamut of biotechnological engineering. Information is given about plasmids and genetic manipulation. After reviewing genetic engineering, this slide show would be useful to recap some of the main procedures.
Curated OER
Biotechnology
The metaphors used here to describe the "cut and paste" procedures for genetic engineering will really help your biologists in their understanding of restriction enzymes and DNA. The uses of genes that are isolated and incorporated into...
Curated OER
Gene Cloning Worksheet #1
For this gene cloning worksheet, students are given a gene sequence of DNA, plasmid DNA, 3 restriction enzymes, their cut patterns and a DNA ligase. They answer 6 questions about the results of using different restriction enzymes to cut...
Curated OER
Who Ate The Cheese?!
In this crime scene investigation worksheet, students simulate DNA sequencing and DNA restriction analysis using paper strips of DNA. Students compare the crime scene DNA to the suspects and determine who is guilty.
Curated OER
Genetic Technology
In this genetic worksheet, students will match 6 terms associated with gene engineering to their correct definition. Then students will complete a table indicating if the vector is mechanical or biological. Finally, students will...
Curated OER
Science Lesson: Chocolate Flavored Cherries
Young scholars are able to identify start and stop sequences in DNA. They are able to model using restriction enzyme and ligase to remove sections of DNA and reattach them. Students are introduced to the process of recombinat DNA...
Curated OER
Simulation of Gene Splicing
Learners use the exercise as a prelude to a "wet" lab or as a substitute for such a lab. It correlates well with colony transformation labs. This lab is recommended for students what have difficulty with the abstractions that genetic...
Curated OER
Science: Designer genes
Students engineer new organisms using biotechnology. In small groups, they write procedures, list benefits and drawbacks, and explain how their new organism might affect the environment. After creating their new organisms, they present...
Curated OER
DNA Race Track
Twelfth graders simulate a DNA fragment running through a gel electrophoresis in a class activity. Students work in teams as a DNA sample with each person on the team representing a fragment that moves through the gel at a specific speed.
Curated OER
DNA Fingerprinting through a Chemistry Lens
Students explore DNA and restriction enzymes using fragments of DNA separated by electrophoresis on an agarose gel. They analyze the results to see "whodunit".
TeachEngineering
Teach Engineering: Dna Forensics and Color Pigments
Students perform DNA forensics using food coloring to enhance their understanding of DNA fingerprinting, restriction enzymes, genotyping and DNA gel electrophoresis. They place small drops of different food coloring ("water-based paint")...
TeachEngineering
Teach Engineering: Restriction Enzymes and Dna Fingerprinting
The discovery of restriction enzymes and their applications in DNA analysis has proven to be essential for biologists and chemists. This lesson focuses on restriction enzymes and their applications to DNA analysis and DNA fingerprinting....
Biology Pages
Kimball's Biology Pages: Restriction Enzymes
This site is a retired biology professor's explanation of restriction enzymes. Good visual showing examples of how enzymes cut DNA to produce blunt or sticky ends.
Cold Spring Harbor Laboratory
Dolan Dna Learning Center: Dna Restriction
Animated slide show demonstrates the genetic engineering process of DNA restriction.
John Wiley & Sons
Interactive Concepts in Biochemistry: Restriction Digestion/gel Electrophoresis
Tutorial explains the processes of restriction digestion and gel electrophoresis and provides an interactive exercise to further your understanding.
Khan Academy
Khan Academy: Restriction Enzymes & Dna Ligase
Learn about restriction digestion, where the result has sticky ends and blunt ends. Also find out about ligation reactions.
Annenberg Foundation
Annenberg Learner: Applied Genetic Modification
Explore practical uses of genetic engineering techniques to generate a plant that produces a hepatitis B protein for potential vaccine use. Examine the use of restriction enzymes for cloning and plasmid mapping.
Khan Academy
Khan Academy: Recombinant Dna Technology
Read a passage about recombinant DNA technology and answer the follow-up questions.
Other
Rebase: Restriction Enzyme Database
Searchable database of restriction enzyme information. Must have more than basic knowledge of restriction enzymes.
ClassFlow
Class Flow: Gel Electrophoresis & Restriction Mapping
[Free Registration/Login Required] Following basic lab techniques of modern molecular biology, analyze the gel electrophoresis results of a restriction enzyme digest of DNA by using a HindIII-generated standard curve. Then solve two...