Curated OER
Genetic Engineering/Biotechnology
Learners create a paper model of a plasmid and insert a DNA molecule that contains a specific gene into the plasmid. They complete an actual transformation involving E. coli bacterium and relate the two activities to one another.
Curated OER
The Cloning of Plasmid and Spinach DNA
Learners will first extract genomic DNA from spinach and perform a restriction enzyme digestion on both the spinach DNA and the plasmid using the same restriction enzyme. They then ligate the cut plasmid DNA with the cut spinach DNA in...
Cold Spring Harbor Laboratory
Harlem Dna Lab: Bacterial Transformations
In this lab, students will transform E. Coli bacteria to be resistant to ampicillin and to glow green. Students will use genetic engineering techniques to insert plasmids into the bacteria. The site contains background reading,...
Estrella Mountain Community College
Estrella Mountain Community College: Plasmids
This site is part of an "Online Biology Book" and contains excellent text and visuals. Discusses F and R plasmids and includes great illustrations. Well organized and clear.
TeachEngineering
Teach Engineering: Bacteria Transformation
Students construct paper recombinant plasmids to simulate the methods genetic engineers use to create modified bacteria.
Cold Spring Harbor Laboratory
Dolan Dna Learning Center: Dna Transformation
Through animated images, follow the experiment which created the first custom-made organism containing recombinant DNA.
Khan Academy
Khan Academy: Overview: Dna Cloning
Explore the definition, purpose, and basic steps of DNA cloning.