Curated OER
Using the Spectrophotometer to Analyze a Mixture
General chemistry classes practice spectroscopy. They grasp the relationships between wavelength, absorbance, and solution concentration. Additionally, they gain valuable practice using laboratory equipment such as burets and pipettes....
Curated OER
The Visible Spectroscopy Expert Witness Problem
In a simulated crime science investigation, chemistry or physics sleuths use spectroscopy to analyze solutions. The lesson gives learners practice making salicylate solutions, using spectrophotometers, calculating dilution amounts,...
Curated OER
Spectrophotometer Determination of an Equilibrium Constant
Students determine an equilibrium constant using a spectrophotometer. For this equilibrium lesson plan, students calibrate a spectrophotometer with 5 solutions in order to prepare the spectrophotometer for the experiment. Students...
Curated OER
Determination of Phosphorus Content in River Water
Divide your chemistry or environmental science class into two groups. Each group tests water samples from a river for the concentration of phosphorus using a different method. With chemists, you can use this activity as they learn to use...
Curated OER
D Block Elements: Complex Ions and Oxidation States
Students investigate transition metals and their oxidation states. In this complex ion and oxidation states lesson plan, students use 6 transition metals and mix them with ligand solutions and oxidizing agents separately. They test their...
Curated OER
Protein Assays: Bio Rad and Page Gels
Young scholars conduct the Biuret Test and the Bio Rad Protein Assay. They examine the spectrophotometer and graph molecular weights and the concentrations of the proteins investigated. They prepare solutions for the Bio-Rad test and use...
Curated OER
Generate a Calibration Curve for Chlorophyll a
High schoolers measure and graph the absorbance of light by chlorophyll at a specific wavelength versus chlorophyll concentration. They are given an unknown and determine its concentration from their calibration curve. Students become...
Curated OER
Operating the spectrophotometer
Working in small groups, learners explore the operation of spectrophotometer. Using the spectrophotometer, they investigate Beer's Law with everyday items.
Howard Hughes Medical Institute
Hhmi: Biointeractive: Virtual Immunology Lab
This virtual laboratory demonstrates how an enzyme-linked immunosorbent assay (ELISA) is carried out and some of the key experimental problems that may be encountered. Students will learn about the assay procedure and the equipment and...
Science Struck
Science Struck: The Calibration Curve
Explains what Beer's Law and a spectrophotometer have to do with calibration curves. Describes the steps in making a calibration curve, by first making a set of standard solutions, then using a spectrophotometer to find the absorbance of...
Davidson College
Davidson College: Spectrophotometry: Basic Principles
Explains the principles of spectrophotometry and how a spectrophotometer works. Presents a simulation that shows how to take spectrophotometric measurements.