Teach Engineering
Restriction Enzymes and DNA Fingerprinting
Show your class why restrictions aren't always a bad thing. In the third segment of a four-part series, the instructor develops the idea of restriction enzymes. Pupils learn how scientists use restriction enzymes in DNA analysis and DNA...
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Biotechnology
The metaphors used here to describe the "cut and paste" procedures for genetic engineering will really help your biologists in their understanding of restriction enzymes and DNA. The uses of genes that are isolated and incorporated into...
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Using a Controlled Experiment to Identify Two Unknown Plasmids
Student demonstrate knowledge of recombinant DNA techniques (restriction enzyme digest, gel electrophoresis, and staining gel). They demonstrate skills needed to complete a gel electrophoresis and interpret a stained gel. Pupils solve...
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From Restriction Maps to Cladograms
Biology aces analyze restriction maps to determine the relationships among different primate species and then complete a cladogram. Preface this lesson with an explanation of how restriction enzymes are used to create the DNA maps....
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Determining Cutting Site Locations
Learners explore DNA restriction mapping. They cut pieces of paper into lengths representing those produced when specific enzymes are used to cut a strand of DNA.
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Enzyme and Southern Blotting
Students study southern blotting and its uses. They research on restriction enzymes and their uses, especially in relation to recombinant DNA. In addition, they write a summary and an interpretation of the gel.
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Science Lesson: Chocolate Flavored Cherries
Students are able to identify start and stop sequences in DNA. They are able to model using restriction enzyme and ligase to remove sections of DNA and reattach them. Students are introduced to the process of recombinat DNA through the...
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Gene Cloning Worksheet #1
In this gene cloning instructional activity, high schoolers are given a gene sequence of DNA, plasmid DNA, 3 restriction enzymes, their cut patterns and a DNA ligase. They answer 6 questions about the results of using different...
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How do Restriction Enzymes Work?
Learners discover how restriction enzymes work, while learning the importance of restriction enzymes in studying DNA structure.
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AP: Chapter 20: DNA Technology
This nine-page biotechnology resource contains six pages of questions regarding DNA technology. Questions cover cloning, restriction enzymes, the polymerase chain reaction, different techniques, the human genome project, and more. The...
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Biotechnology
The concepts here cover the gamut of biotechnological engineering. Information is given about plasmids and genetic manipulation. After reviewing genetic engineering, this slide show would be useful to recap some of the main procedures.
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Forensic Files: A DNA Dilemma
Seventh-graders come in to science class to find a file on their desks detailing a crime to be solved! As a demonstration, you simulate the restriction of DNA samples and separate them by electrophoresis. From the gel, learners can...
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Restriction Enzymes
Learners explore enzymes and enzyme combinations. They examine and cut up and practice matching DNA sequences.
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Who Ate The Cheese?!
In this crime scene investigation worksheet, students simulate DNA sequencing and DNA restriction analysis using paper strips of DNA. Students compare the crime scene DNA to the suspects and determine who is guilty.
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Simulation of Gene Splicing
High schoolers use the exercise as a prelude to a "wet" lab or as a substitute for such a lab. It correlates well with colony transformation labs. This lab is recommended for students what have difficulty with the abstractions that...
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DNA Fingerprinting through a Chemistry Lens
Students explore DNA and restriction enzymes using fragments of DNA separated by electrophoresis on an agarose gel. They analyze the results to see "whodunit".
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Innocent or Guilty: A Lab on DNA Gel Electrophoresis
Students use the prelab as an introduction to the importance of DNA fingerprinting- a form of identification that us being accepted by both scientific and leagl experts. They prepare a gel for electrophoresis. DNA fragments, which have...
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Genetic Technology
In this genetic learning exercise, students will match 6 terms associated with gene engineering to their correct definition. Then students will complete a table indicating if the vector is mechanical or biological. Finally, students will...
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DNA Fingerprinting with Restriction Enzymes
Learners will illustrate how restriction enzymes work by piecing together parts of a puzzle. Then they apply this knowledge to help them analyze genetic evidence from the scene of a crime as well as to help them comprehend how a DNA...
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Cut-off Genes
Investigate the relationships between different deep-sea organisms by DNA sequencing. A worksheet provides instructions for DNA sequencing and space to work. They simulate gel electrophoresis by cutting out paper "DNA strands." Modeling...
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The Clark Family Story: Tracing the Cause of Hemophilia
Ms. Strohfeldt did not omit anything when she designed this comprehensive lesson plan on DNA mutation and sex-linked traits. Begin with a pretest as an anticipatory set. Read a case history of the Clark family and the occurrence of...
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Fly Forensics
Freddy's Friendly Fruit Market is under attack by fruit flies, and it is up to your aspiring biologists to solve the crime! By reading and analyzing the phenotypes, detectives determine which fly is responsible. This memorable assignment...
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Restriction Digest + DNA Comparison
High schoolers compare different DNA and run a gel electrophoresis. For this DNA lesson students complete a DNA activity that allows them to better understand its structure and function.
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lambda DNA Fingerprinting Simulation
Students perform restriction digests on DNA samples from four individuals, and then search for similarities between the individuals by running the restriction fragments on an electrophoresis gel.