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Activity
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Teach Engineering

DNA Forensics and Color Pigments

For Teachers 9th - 12th
Use food coloring in electrophoresis. The last segment in a four-part series mimics DNA fingerprinting by using chromatography. Teams conduct chromatography on food coloring to find colors that use similar pigmentation in their makeup.
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Lesson Plan
Curated OER

DNA Recombination II

For Teachers 9th - 10th
Students model recombination in DNA. They color the DNA sample and cut it out. Students use their "restriction enzymes" to cut the DNA into specific fragments. They separate the fragments using the "gel electrotrophoresis." Students...
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Lesson Plan
Curated OER

Gel Electrophoresis: Sort and See the DNA

For Teachers 8th - 12th
Learners construct DNA fingerprints. In this genetics activity, students watch an on-line gel electrophoresis animation, then they work in groups to use a restriction map to create a restriction enzyme and use that to arrange a DNA...
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Lesson Plan
1
1
Teach Engineering

Imagining DNA Structure

For Teachers 9th - 12th
Let's get a closer look at DNA and other molecular structures. The first lesson in the series of four introduces a variety of imagining techniques that engineers and scientists use to visualize molecular structures. The resource presents...
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Lesson Plan
Curated OER

cDNA Sequencing By Restriction Project

For Teachers 9th - 12th
Young scholars try to confirm the sequence of a given cDNA clone using the WWW tools and traditional restriction analysis. They grow the clone, prep the plasmid, cut the plasmid with restriction enzymes and compare their results to what...
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Lesson Plan
Curated OER

Determination of Phylogenetic Relationships Using Restriction Analysis

For Teachers 9th - 10th
Students applied various mathematical analyses to interpret their data. By using the resulting DNA fragment patterns from several enzymes students established a possible evolutionary relationship among the mice.
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Lesson Plan
Curated OER

Molecular Explorations

For Teachers 9th - 12th
Students explain the processes for separating DNA from tissue and separating complex mixtures.  In this exploratory lesson plan students complete a lab where they extract DNA.  
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Organizer
Curated OER

Gel Electrophoresis: Sort and See the DNA

For Students 7th - 12th
In this DNA worksheet, students access a website to learn how DNA fragments are separated through the process of gel electrophoresis. Then students model the construction of DNA fingerprints and practice interpreting restriction maps....
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Lesson Plan
Curated OER

Science: Designer genes

For Teachers 9th - 12th
Students engineer new organisms using biotechnology. In small groups, they write procedures, list benefits and drawbacks, and explain how their new organism might affect the environment. After creating their new organisms, they present...
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Lesson Plan
Curated OER

DNA Race Track

For Teachers 12th
Twelfth graders simulate a DNA fragment running through a gel electrophoresis in a class activity. Students work in teams as a DNA sample with each person on the team representing a fragment that moves through the gel at a specific speed.
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Lesson Plan
Curated OER

DNA Fingerprinting for High School Biology

For Teachers 9th - 10th
Students work with tools and techniques used in genetic engineering. They dissect a sample into fragments of DNA using restriction enzymes, sometimes called "molecular scissors". They sort these fragments, measure and analyze them according
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Lesson Plan
Curated OER

Characterization of the Bioluminescence (Lux+) Gene E. coli

For Teachers Higher Ed
Students grow E. coli strain HB101, which contains the plasmid pUCD607 with the bioluminescence (Lux+) gene. The plasmid containing the Lux+ gene is isolated from the E. coli, then characterized by restriction analysis.
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Lesson Plan
Curated OER

Gel Electrophoresis

For Teachers 9th - 12th
Young scholars conduct a variety of experiments to explore gel electrophoresis. In this biology lesson, explain how this process separate DNA and RNA. They discuss the practical applications of this method.
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Lesson Plan
Towson University

Case of the Crown Jewels

For Teachers 9th - 12th Standards
Can your biology class crack the Case of the Crown Jewels? Junior forensics experts try their hands at DNA restriction analysis in an exciting lab activity. The lesson introduces the concept of restriction analysis, teaches pipetting and...
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Lesson Plan
Curated OER

COMPARISON OF FOUR-, SIX-, AND EIGHT-bp CUTS IN CALF THYMUS DNA

For Teachers 9th - 12th
Learners use this laboratory exercise, restriction enzymes to recognize a four-, six-, and eight-nucleotide sequence. Assuming that the four component nucleotides (A, C, T, G) are distributed randomly within a DNA molecule, then any...
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Interactive
Cold Spring Harbor Laboratory

The RNA Message Is Sometimes Edited

For Students 9th - 12th Standards
In 1993, Phillip Sharp and Richard Roberts won the Nobel Prize for their discovery of split gene theory. Learn about the breakthrough with the help of an online interactive. Hear both scientists explain it in their own words, watch an...
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Worksheet
Curated OER

DNA Technology

For Students 9th - Higher Ed
In this DNA technology worksheet, students will review 12 vocabulary words relating to the uses and results of DNA technology in this crossword puzzle.
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Lesson Plan
Curated OER

Comparison of Four-, Six-, And Eight-bp Cuts in Calf Thymus Dna

For Teachers 9th - 12th
Students investigate the use of restriction enzymes to recognize a four-, six-, and eight-nucleotide sequence. They utilize restrictive endonucleases to cut Thymus DNA and compare results by viewing the DNA fragments on Agarose Gel...
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Lesson Plan
Curated OER

The Cloning of Plasmid and Spinach DNA

For Teachers 10th - 12th
Students will first extract genomic DNA from spinach and perform a restriction enzyme digestion on both the spinach DNA and the plasmid using the same restriction enzyme. They then ligate the cut plasmid DNA with the cut spinach DNA in...
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Lesson Plan
Curated OER

DNA Fingerprinting

For Teachers 9th
Ninth graders investigate what restriction enzymes do, and explain how a DNA sample can link a suspect to a crime.
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Lesson Plan
Curated OER

Cloning of Plasmid and Spinach DNA

For Teachers 12th - Higher Ed
Students ligate fragments of genomic DNA from spinach into a vector plasmid. They perform a restriction enzyme digestion. Student use a ligated DNA molecule to transform E. coli cells. Students analyze their procedures and outcome of...
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Lesson Plan
Curated OER

From Restriction Maps to Cladograms

For Teachers 9th - 12th
Students develop a matrix to determine the number of differences between each set of primate species. They determine closely related organisms then complete the cladogram and answer a set of questions.
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Lesson Plan
Curated OER

Gel Electrophoresis Simulation

For Teachers 9th - 10th
Learners identify the steps in a gel electrophoresis experiment and describe the role of enzymes. They examine the relationship between fragment size and migration rate in a gel. They view DNA fragments as well.
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Lesson Plan
Curated OER

Simulation of Gene Splicing

For Teachers 10th - 12th
Students simulate gene splicing by examining bacteria. They create a bacterial DNA into which they insert the human DNA (gene) that codes for growth hormone. They cut the sites of the enzyme to study how the human DNA can be joined to...

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